Skip to main content
. 2024 Jun 18;13(6):514. doi: 10.3390/pathogens13060514

Figure 1.

Figure 1

Gating strategy for subsets of CD4+ T cells. Contour plot was used for gating strategy; gated lymphocytes were analyzed for singlet and CD4-expressing cells. These CD4+ cells were further analyzed for (A) CD4 subsets naïve: CCR7+CD45RA+, central memory (CM): CCR7+CD45RA−, effector memory (EM): CCR7−CD45RA−, and T effector memory: CD45RA+ (TEMRA) CCR7− CD45RA+. (B) Treg CD25+CD127 low cells expressing FoxP3. (C) Follicular helper cells (TFH) CXCR5+CD45RA−; subsets of TFH cells were further analyzed—TFH1: CXCR3+CCR6−, TFH2: CXCR3−CCR6−, TFH17: CXCR3−CCR6+.