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. 2024 Jun 18;13(6):514. doi: 10.3390/pathogens13060514

Figure 2.

Figure 2

Gating strategy for subsets of CD8+ T cells. Contour plot was used for gating strategy; gated lymphocytes were analyzed for singlet and CD8-expressing cells. These CD8+ cells were further analyzed for (A) CD4 subsets naïve: CCR7+CD45RA+, central memory (CM): CCR7+CD45RA−, effector memory (EM): CCR7−CD45RA−, and T effector memory RA+ (TEMRA): CCR7−CD45RA+. (B) CD8Treg CXCR3 expressing CM: CCR7+CD45RA−. (C) FoxP3 and ICOS+ expression in CD25+CXCR3+ CD8 cells. (D) CD8+ cells were analyzed for granzyme B and perforin expression. (E) SARS-CoV-2 spike protein-specific, tetramer-positive cells in total CD8 and subsets.