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Gating strategy for subsets of CD20+ B cells. Contour plot was used for gating strategy; gated lymphocytes were analyzed for singlet and CD20-expressing B cells. These B cells were further analyzed for naïve: IgD+CD27−, MZ: IgD+CD27+, CSM: IgD−CD27+, IgM memory: IgM+CD27+, transition B cells: IgM+CD38+, plasma blast: IgM−CD38+, CD21High and CD21Low, Breg: CD24+CD38+, and GC cells: IgD−CD38+CD27−.
