Figure 7.

Effect of in vitro activation with SARS-CoV-2 protein on activation of CD4 and CD8 T cells and IFNγ+ CD4 and CD8 T cells. PBMC were activated with SARS-CoV-2 spike protein, activation of CD4 and CD8 was examined by the expression of CD69 and CD137, and intracellular IFNγ was examined in CD69+ and CD137+ CD4 and CD8 T cells. A representative contour flow cytograph for HC and PAD is shown (A). Cumulative data show significantly increased CD8+CD69+ T cells and CD4+CD137+ T cells and significantly decreased C4+CD137+IFNγ+ T cells in the PAD group (B).