TABLE 2.
Comparison of relative luciferase activities of pJS21-luc in selected cell lines by using different reference promoters and/or cotransfected plasmids
| Cell line | Avg relative luciferase activity ± SEM (95% CI)a
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Set 1
|
Set 2
|
Set 3 (pJS21luc/pRL-tk ratio)f | Set 4 (pJS21luc/pRL-null ratio)f | |||||||
| SVb | CMVc | MLVd | Basice | SV | CMV | MLV | Basic | |||
| MLE-15 | 223 ± 19.5 | 9.7 ± 1.2 | 22.8 ± 0.1 | 11.2 ± 1.4 | 187 ± 5.4 | 52.1 ± 0.8 | 50 ± 1 | 11.6 ± 0.22 | 77.2 ± 1.3 | 56.1 ± 1.9 |
| mtCC1-2 | 51.3 ± 7.5 | 15.2 ± 3.2 | 22.7 ± 5 | 12.4 ± 1.6 | 90 ± 2.4 | 93.6 ± 2.4 | 76.3 ± 3 | 5.6 ± 0.4 | 62.9 ± 0.8 | 29.9 ± 0.6 |
| NIH 3T3 | 5.9 ± 0.4 | 2.4 ± 0.2 | 8.8 ± 0.4 | 4.5 ± 0.4 | 5.9 ± 0.1 | 46 ± 1.2 | 24 ± 0.5 | 2.5 ± 0.1 | 3.3 ± 0.1 | 5.4 ± 0.1 |
| TCMK | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| ST3 | 2.9 ± 0.3 | 3.1 (0.3) | 14.3 ± 1.2 | 0.8 ± 0.1 | 6.5 ± 0.2 | 15 ± 1.1 | 34.7 ± 1.4 | 1 ± 0.1 | 11.6 ± 1.5 | 6.8 ± 0.1 |
| CP-MRI | 7.1 (1.6) | 6.6 ± 1 | 4 (0.3) | 13.2 ± 1 | 29.2 ± 1 | 46.2 ± 3 | 34 ± 1.1 | 9.4 ± 0.1 | 26.8 ± 1.5 | 76.5 ± 2.5 |
| CP-ATCC | 2.2 ± 0.1 | 1.8 ± 0.2 | 0.9 ± 0.1 | 6.1 ± 1 | 6.6 ± 0.1 | 10.4 (0.3) | 9.9 ± 0.3 | 3.5 ± 0.2 | 24 ± 0.1 | 13 ± 1 |
Results were expressed by taking as a value of 1 (in italics) the activity of pJS21-luc relative to each neutral promoter in TCMK cells. CI, confidence interval. Experiments in sets 1 and 2 also included cotransfections of each luciferase reporter (e.g., pJS21-luc) with a second plasmid (pCMV-βgal, set 1; pRL-tk, set 2) to allow normalization for DNA transfection efficiency among different experiments. The values shown are averages of at least six independent experiments. In boldface are the two highest values in each series of experiments.
SV, pGL3-control (SV40 promoter-enhancer).
CMV, pCMV-luc.
MLV, pMLV-luc.
Basic, pGL3-basic (promoterless luciferase plasmid).
All cotransfections contained 500 ng of pJS21-luc/well and 50 ng of pRL-tk or pRL-null/well.