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Twelve females were isolated after blood feeding and monitored for eggs laid (A), hatching (B), development to adulthood (C) and differentiation to males (♂) and females (♀) (D). Error bars are medians with 95% confidence intervals. One-way ANOVA analysis was performed to obtain the P values. Values of P > 0.05 were considered not statistically significant (ns) and P ≤ 0.01 are statistically significant (**).
