Table 1. Primers for synthesis of sgRNA, PCR and RT-PCR.
Primer | Sequence 5’➔3’ |
---|---|
sgRNA-55rv | GAAATTAATACGACTCACTATAGGCTGCTCCCATTCCCCTGGGTTTTAGAGCTAGAAATAGCAAG |
sgRNA11-4fw | GAAATTAATACGACTCACTATAGGTCCCGGAAAAGGTCACCGTGTTTTAGAGCTAGAAATAGCAAG |
sgRNA-23fw | GAAATTAATACGACTCACTATAGGACTTTGTATCGGTCTCGCGGTTTTAGAGCTAGAAATAGCAAG |
sgRNA-124rv | GAAATTAATACGACTCACTATAGGCCTGCTGGTTCCAATAGCTGTTTTAGAGCTAGAAATAGCAAG |
T7common | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
-0.6 CAD F | GGTGGAATTCTCCTTGGAAACATTT |
+0.3 CAD R | CCCTCACTGTACATCGATGGC |
-0.3 mALP F | GACTACTTCGGAACATAGGGCAAC |
+0.3 mALP R | CTCTGGGCCTCGACAGTTAAG |
CAD-F Exon3 | ATAGCGGTCACTATTCCTGC |
CAD-R Exon3 | CATTGAAACTTCCTGTTCGCG |
Nix F1 | GATTTTTGTTTTTGTCGTGCAA |
Nix R1 | AATGCAAGTATCATAGGTCAGCA |
rps3Ae Fwd | GGCATGTTCCGTGCTGAATTGAACG |
rps3Ae Rev | TTCTCGGCGTACAGCTCGACG |