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. 2024 Jun 13;18(6):e0012256. doi: 10.1371/journal.pntd.0012256

Table 1. Primers for synthesis of sgRNA, PCR and RT-PCR.

Primer Sequence 5’➔3’
sgRNA-55rv GAAATTAATACGACTCACTATAGGCTGCTCCCATTCCCCTGGGTTTTAGAGCTAGAAATAGCAAG
sgRNA11-4fw GAAATTAATACGACTCACTATAGGTCCCGGAAAAGGTCACCGTGTTTTAGAGCTAGAAATAGCAAG
sgRNA-23fw GAAATTAATACGACTCACTATAGGACTTTGTATCGGTCTCGCGGTTTTAGAGCTAGAAATAGCAAG
sgRNA-124rv GAAATTAATACGACTCACTATAGGCCTGCTGGTTCCAATAGCTGTTTTAGAGCTAGAAATAGCAAG
T7common AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC
-0.6 CAD F GGTGGAATTCTCCTTGGAAACATTT
+0.3 CAD R CCCTCACTGTACATCGATGGC
-0.3 mALP F GACTACTTCGGAACATAGGGCAAC
+0.3 mALP R CTCTGGGCCTCGACAGTTAAG
CAD-F Exon3 ATAGCGGTCACTATTCCTGC
CAD-R Exon3 CATTGAAACTTCCTGTTCGCG
Nix F1 GATTTTTGTTTTTGTCGTGCAA
Nix R1 AATGCAAGTATCATAGGTCAGCA
rps3Ae Fwd GGCATGTTCCGTGCTGAATTGAACG
rps3Ae Rev TTCTCGGCGTACAGCTCGACG