RhoGDI2 metastasis suppressor activity is modulated by post-translational mechanisms. RhoGDI2 can be phosphorylated on Tyr 156 by Src, and this phosphorylation causes dissociation of Rac1 and RhoGDI2, re-localization of both Rac1 and RhoGDI2 to membranes, a corresponding increase in Rac1 activation, and enhanced metastasis suppressor activity. Alternately, RhoGDI2 can be phosphorylated on Ser 31 by PKCα which causes dissociation of Rac1 and RhoGDI2, activation of Rac1, and enhanced metastasis