FIG. 1.

Resistance of RNA polymerase activity in crude oocyst lysates. ³²P-labeled products of RNA polymerase assays were resolved by electrophoresis on 0.8% agarose gels (A and B) or on a 6% polyacrylamide gel (C) under nondenaturing conditions and were then exposed to X-ray film. (A) Assays were performed in the presence of different concentrations of proteinase K. (B) Aliquots were incubated for 30 min at 37°C with different concentrations of RNase A, then RNase was destroyed by treatment with proteinase K (0.5 mg/ml, 30 min at 37°C), and finally [α-³²P]UTP was added and the mixture was incubated for 1 h at 37°C. (C) RNA polymerase mixtures were untreated, incubated with 0.1% SDS or 0.1% SDS–0.5 mg of proteinase K per ml, or extracted once with phenol-chloroform and then loaded on the gel.