FIG. 4.

Distribution of proteins encoded by ORFs of the L- and S-dsRNAs in CsCl gradient fractions. (A) Expression of the ORFs of the L- and S-dsRNAs in E. coli. Shown is a Coomassie blue-stained SDS–10% polyacrylamide gel with total proteins from uninduced (−IPTG) and induced (+IPTG) E. coli cells and affinity-purified recombinant proteins. Arrows show positions of recombinant proteins. Molecular size standards are shown in kilodaltons in the right margin. (B and C) Immunoblot analysis of the proteins from the CsCl fraction using antibodies raised against recombinant proteins of L-dsRNA (B) and S-dsRNA (C). (D) Coomassie blue-stained SDS–10% polyacrylamide gel with the proteins from CsCl fractions. Molecular size standards are shown in kilodaltons in the right margin. Only fractions 5 to 18 out of 30 total are shown.