Effects of phospholipids on social interaction. (A) Three hours after trial 1, the social interaction time of male rats with an intruder was measured in trial 2. Rats were reintroduced in the same context as in trial 2, 21 h after the first exposure to the familiar intruder rat, in trial 3. Three hours later, male rats were tested for interaction with a novel intruder in trial 4. (B,C) Social interaction. (D,E) Object interaction. (F–H) Discrimination ratios. Values are presented as the mean ± standard error. The rat numbers in each group were as follows: (B,D) Egg PC (n = 8), PE(18:018:0) (n = 8), PE(18:0/22:6) (n = 8), PE(18:0/20:4) (n = 9), and PE(P-18:0/20:4) (n = 6). (C,E) Egg PC (n = 10), PC(16:0/22:6) (n = 10), PC(O-16:0/22:6) (n = 6), PC(O-18:0/22:6) (n = 9), and PE(O-16:0/22:6) (n = 3). Differences were evaluated using the Tukey–Kramer HSD test (B–E) and Hsu’s MCB test (F–H). * p < 0.05, ** p < 0.01.