TABLE 4.
Percent enhancement in recognition by CD4-IgG of primary viral isolates following heat treatmenta
| Virus | % Enhancement (mean ± SE) |
|---|---|
| LTS | |
| 1 | 440 ± 155 |
| 3 | 147 ± 19 |
| 4 | 237 ± 84 |
| 5 | 122 ± 18 |
| 8 | 233 ± 57 |
| Seroconverter 13 | 369 ± 176 |
a
Data are expressed as percent gp120 binding to CD4-IgG at 62°C relative to binding at 4°C. Aliquots of viral isolates were held at 4°C or heated to 62°C for 30 min. Binding using the indicated reagents was performed for 45 min at 37°C in 200 μl. The retention of epitopes was assessed by HIV gp120 capture ELISA. Control reactivity without Ag was <0.1. CD4-IgG was synthesized in 293T cells and used at a 1:10 dilution. The data are the means from three independent experiments (each experiment was run in triplicate).