Figure 5.

Piperlongumine affects keratinocyte aging markers and telomerase activity. HaCaT cells were treated with 1 μM piperlongumine at the indicated oxygen concentration. (A) After incubation for 48 h, real-time PCR was performed to determine the mRNA expression of genes associated with the skin barrier function. ACTB was used as a normalization control. H2AJ; H2A.J Histone, ITGA6; Integrin Subunit Alpha 6. (B) After incubation for 48 h, cell lysates were prepared to determine the activity of telomerase. The number of telomerases per cell represents the enzyme with activity after piperlongumine treatment. GraphPad Prism was used to prepare the graphs, and statistical significance was determined using one-way ANOVA. ns; non-significant, **; p < 0.01, ***; p < 0.005, ****; p < 0.001