Table 5.
Demethylation of 5mCpG in promoter regions of chemical carcinogenesis-receptor activation pathway
| Gene Name | DMR Location in Promoter Region |
DMR (Methylation %) |
|||||
|---|---|---|---|---|---|---|---|
| Chromosome | Start | End | 3 h | 6 h | 12 h | 24 h | |
| BCL6 | Chr24 | 187,455,336 | 187,455,954 | −20.42 | −43.30 | −33.25 | −31.52 |
| CACNA1D | Chr7 | 53,529,276 | 53,529,335 | −55.12 | −69.10 | 0.00 | 0.00 |
| CREB3L4 | Chr5 | 153,938,859 | 153,938,897 | 0.00 | −36.38 | 0.00 | −35.93 |
| CYP1A1 | Chr5 | 75,019,335 | 75,019,387 | −30.07 | −55.38 | 0.00 | −27.04 |
| ESR2 | Chr14 | 64,751,715 | 64,751,757 | 0.00 | −33.80 | −28.97 | −24.69 |
| FGF8 | Chr10 | 103,539,541 | 103,539,685 | −19.97 | −53.29 | −37.87 | 0.00 |
| MIR29A | Chr7 | 130,562,558 | 130,562,743 | −15.46 | −32.98 | −22.09 | −26.93 |
| MIRLET7B | Chr22 | 46,508,476 | 46,508,565 | 0.00 | −34.85 | 0.00 | −24.75 |
| MTOR | Chr1 | 11,323,660 | 11,323,854 | −20.34 | −37.30 | −27.13 | 0.00 |
Gene name represent the official gene symbol as recorded in NCBI database. Differentially methylated region (DMR) (methylation %) represents the percentage change in methylation levels induced by cholestenoic acid (CA) treatment at various time points compared with the control group. P = 0.0027 at 6 h.