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. 2024 Jun 26;13:RP94605. doi: 10.7554/eLife.94605

Figure 6. Inhibition of ERK phosphorylation stabilizes LGR4 through downregulating NEDD4.

Figure 6.

(A) Immunoblotting analysis of cecum lysates from indicated 6-week-old mice. (B) Immunoblotting analysis of cecum lysates from 6-week-old BC mice treated with vehicle or MEK inhibitor PD0325901. MEK inhibitor was given to the mice at a dose of 25 mg/kg three times at 12 hr intervals. Twenty-eight hours after the first treatment, the cecum mucosa was collected for immunoblotting. (C) Immunoblotting analysis of lysates from HT-29 cells treated with DMSO or 10 µM MEK inhibitor for 24 hr. (D) HT-29 cells were transfected with HA-Ubiquitin. One day later, the cells were treated with DMSO or 10 µM MEK inhibitor for 24 hr. Then all the cells were incubated with 10 µM MG132 for additional 4 hr. The cell lysates were collected for immunoprecipitation and immunoblotting with the indicated antibodies.

Figure 6—source data 1. Uncropped and labelled gels for (Figure 6).
Figure 6—source data 2. Raw unedited gels for (Figure 6).