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. 2024 Jun 26;13:RP94605. doi: 10.7554/eLife.94605

Figure 7. ERK activation is FAK/EGFR-independent in KC mice.

Figure 7.

(A) Representative hematoxylin and eosin (H&E) staining of the small intestine, cecum, and colon from indicated 9-month-old mice. (B) Immunoblotting analysis of intestinal mucosa lysates from indicated bowel subsites in indicated 6-week-old mice. (C) The cecal mucosa lysates from 6-week-old KC and BC mice were used for immunoprecipitation and immunoblotting with the indicated antibodies. (D) Immunoblotting analysis of cecum lysates from 6-week-old KC mice treated with vehicle or EGFR inhibitor erlotinib for 4 hr. (E and F) Comparison of FAK expression levels between CRCs with indicated mutations by analysis of TCGA RNA-sequencing dataset. Data were analyzed for statistical significance using a Student t-test. (G) Diagram of the ‘just-right’ MAPK signaling model in the serrated pathway.

Figure 7—source data 1. Uncropped and labelled gels for (Figure 7).
Figure 7—source data 2. Raw unedited gels for (Figure 7).