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. 2024 Apr 25;18(2):e12029. doi: 10.1002/ccs3.12029

FIGURE 3.

FIGURE 3

CircORC2 upregulated TRIM2 expression by targeting miR‐485‐3p. (A). The Circinteractome database was used to predict the binding relationship between circORC2 and miR‐485‐3p. (B). The luciferase activity was evaluated via the dual‐luciferase assay. (C). Quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) was employed to detect the miR‐485‐3p level after transfecting the U‐2Os and SJSA‐1 cells with sh‐circORC2 and oe‐circORC2. (D). The starBase database was used to predict the binding sites between miR‐485‐3p and TRIM2. (E). The luciferase activity was evaluated using the dual‐luciferase assay. (F). TRIM2 levels detected by qRT‐PCR after transfecting the U‐2Os and SJSA‐1 cells with miR‐485‐3p mimics and miR‐485‐3p inhibitor. Data are presented as mean ± SD of three replicate experiments (n = 3), **p < 0.01 and ***p < 0. 001.