Extended Data Fig. 9. TrkBcKO mice have normal hairy skin mechanosensitivity, and TrkBcKO males display deficits in mating behaviours.
a, Summary of the percent inhibition of the startle response to an acoustic stimulus (125 dB, 20 ms) caused by a light air puff (prepulse, 0.9 PSI, 50 ms) applied to the back hairy skin preceeding the acoustic stimulus at various interstimulus intervals, for both female (left) and male (right) TrkBcKO mice (n = 10 for females, 8 for males) and control littermates (n = 10 for females, 8 for males; error bars refer to s.e.m.). Multiple unpaired t-test for each interstimulus interval, no significant differences for all interstimulus intervals. b, The total number of bouts of scratching, shaking, or biting a piece of tape stuck to back hairy skin of mice within a 5 min period, for both female (left) and male (right) TrkBcKO mice (n = 8 for females, 10 for males) and control littermates (n = 9 for females, 10 for males). Unpaired t-test, p = 0.79 for females, p = 0.70 for males. c, Ethograms of mating behaviour of TrkBcKO (n = 8) and littermate control (n = 9) males within a 75-minute testing period across three trials, with trials separated by at least one week. The sniffing bouts shown are limited to the male’s sniffing behaviour before the first mount. Mounting refers to shallow probing and postural maneuvering without gaining access to the female genitalia, while intromission represents periods of time during which long rhythmic thrusts are observed. d, Quantification of mounting behaviours across three trials. Unpaired t-test, ** p < 0.01. e, Quantification of ejaculation behaviours, collected across three trials. Only animals that achieved ejaculation are included. f, Quantification of intromission behaviours across three trials. Unpaired t-test, * p < 0.05, **** p < 0.0001 (for intromission start time, * p = 0.029; for intromission total duration, * p = 0.014; for inter-intromission interval, * p = 0.028). g-i, Comparison of intromission start time (g), inter-intromission interval (h), and ejaculation start time (i) within each trial. Unpaired t-test, * p < 0.05. All error bars from b-i refer to s.d.