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. 2024 Jun 19;630(8018):926–934. doi: 10.1038/s41586-024-07528-4

Fig. 3. Krause corpuscle afferents project to the dorsomedial spinal cord.

Fig. 3

a,b, Retrograde labelling of DRG neurons innervating the clitoris (a) or penis (b) and the adjacent midline hairy skin region using CTB tracers conjugated to different fluorophores (hairy: CTB-488; genital: CTB-546). Coronal sections of the upper sacral spinal cord are shown on the right. c,d Labelling of Krause corpuscle afferents using injection of AAV2-retro-hSyn-FlpO into the clitoris (c) or glans penis (d) of TrkBcreER;R26FSF-LSL-Tdtomato (tamoxifen, 0.5 mg at P5) or RetcreER;R26FSF-LSL-TdTomato (tamoxifen, 3 mg at E12.5) mice, with IB4 staining to label the lamina II of the spinal cord as a reference for laminar location. Similar results were observed in more than three animals per condition. For ad, scale bars, 200 µm. The diagrams in a and b were created by G. Park.