Extended Data Fig. 3. Krause corpuscles are dually innervated by TrkB⁺ and Ret⁺ fibres, and TrkB is required for formation of Krause corpuscles.

a, Quantification of the fraction of corpuscles innervated by NF200⁺ axons labelled using TrkB^CreER; Avil^FlpO; R26^{FSF-LSL-Tdtomato} or Ret^CFP mice, among all S100⁺ corpuscles in a given section (TrkB: 10 sections from three males and four sections from two females; Ret: 10 sections from two males and 15 sections from three females). b, Quantification of the overlap of TdTomato and CFP in DRG soma of TrkB^CreER; Ai14; Ret^CFP mice. c, Dual labelling of Ret⁺ and TrkB⁺ Krause corpuscle afferents showing dually innervated corpuscles and a simple corpuscle innervated only by a TrkB⁺ fibre (bottom) stained for tdTomato (TrkB⁺), CFP (Ret⁺), and S100. Scale bar is 10 µm in the first row and 50 µm in all other rows. d, Representative density heatmaps and immunofluorescence images of 200 µm-thick sections of the clitoris and glans penis of wild type and TrkB^cKO animals stained with NF200 and S100. In density heatmaps, the border of the section is outlined in white. The same colour scale, normalized by the densest area of the clitoris, is used for all four heatmaps. The regions indicated by rectangles in the immunofluorescence images are magnified in the bottom. Similar results were observed in more than 3 animals for each condition. Scale bar is 200 µm in d.

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