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. 2024 Jun 26;630(8018):994–1002. doi: 10.1038/s41586-024-07570-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — (a, b) Structures of TBL–tDNA (a) and DBL–dDNA (b). (c, d) Recognition of the CT core sequences in tDNA (c) and dDNA (d). (e, f) Recognition of the CT core-complementary sequences in tDNA (e) and dDNA (f). (g) Modeling of dG8 into the dDNA as part of the core dinucleotide. (h) Interaction between the DNA and the hydrophobic wedge. Similar interactions are observed in the four protomers. In (a–f) and (h), cryo-EM density maps are shown as grey semi-transparent surfaces. (i) Schematic of the bacterial recombination assays. Successful recombination between pTarget and pDonor places the gene encoding green fluorescent protein (GFP) downstream of the synthetic promoter, resulting in fluorescence. IS621, the IS621 recombinase. (j) DNA recombination activities of the WT IS621 and the hydrophobic-wedge mutants in the bacterial recombination assays. Data are shown as mean ± SD for three biological replicates. dWED, Y264A/M265A/M268A.