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. 2024 Jun 26;630(8018):984–993. doi: 10.1038/s41586-024-07552-4

Fig. 3. The IS621 target site is reprogrammable and is specified by the bridge RNA.

Fig. 3

a, Schematic representation of the plasmid recombination assay with bridge RNA in cis. b, GFP fluorescence of E. coli after DNA recombination of the plasmid reporter system using catalytic variants of the IS621 recombinase. Plots are representative of three replicates. c, Schematic of reprogrammed target and bridge RNA target-binding loop sequences. d, GFP mean fluorescence intensity (MFI) of E. coli after plasmid recombination using the indicated reprogrammed bridge RNA target-binding loop and target sequences (WT and T1–T7). Bold bases highlight differences relative to the WT target sequence. Mean ± s.d. of three biological replicates. e, Schematic of bridge RNA expression in trans. f, Comparison of recombination efficiency with bridge RNA expressed in cis and in trans. Mean ± s.d. of three biological replicates.