Table 4.
Physicochemical properties of NE-mRNA nanocarriers, using different types of mRNAs and different N/P ratios
| Code | Type of mRNA | Formulation method | N/P ratio | Size (nm) | PDI | ζ-Potential (mV) | EE (%) |
|---|---|---|---|---|---|---|---|
| NE-3 | mGFP | Bulk mixing | 2:1 | 108 ± 13 | 0.14 ± 0.03 | + 39 ± 2 | 100 |
| NE-4 | mGFP | Bulk mixing | 2:1 | 127 ± 13 | 0.09 ± 0.03 | + 43 ± 4 | 100 |
| mLuc | Bulk mixing | 2:1 | 138 ± 18 | 0.14 ± 0.06 | + 42 ± 2 | 100 | |
| mLuc | Bulk mixing | 4:1 | 89 ± 2 | 0.21 ± 0.02 | + 48 ± 6 | 100 | |
| mLuc | Microfluidics | 4:1 | 84 ± 11 | 0.21 ± 0.02 | + 54 ± 3 | 100 | |
| mOVA | Bulk mixing | 2:1 | 121 ± 9 | 0.10 ± 0.01 | + 40 ± 3 | 100 | |
| NE-5 | mGFP | Microfluidics | 4:1 | 62 ± 13 | 0.24 ± 0.07 | + 53 ± 7 | 100 |
| NE-9 | mGFP | Bulk mixing | 4:1 | 97 ± 1 | 0.19 ± 0.02 | + 51 ± 3 | 100 |
| mLuc | Bulk mixing | 4:1 | 104 ± 1 | 0.17 ± 0.01 | + 52 ± 2 | 100 | |
| NE-10 | mGFP | Microfluidics | 4:1 | 65 ± 1 | 0.2 ± 0.01 | + 44 ± 1 | 100 |
| NE-11 | mGFP | Bulk mixing | 4:1 | 107 ± 3 | 0.18 ± 0.02 | + 53 ± 2 | 100 |
| NE-12 | mGFP | Bulk mixing | 4:1 | 123 ± 2 | 0.17 ± 0.01 | + 49 ± 1 | 100 |
| NE-13 | mOVA | Microfluidics | 2:1 | 116 ± 1 | 0.17 ± 0.01 | + 49 ± 1 | 75 |
Abbreviations: EE: encapsulation efficiency. mGFP: mRNA encoding for GFP. mLuc: mRNA encoding for luciferase. mOVA: mRNA encoding ovalbumin protein. NE: nanoemulsion. N/P ratio: nitrogen to phosphate ratio. PDI: polydispersity index. Values represent the mean ± standard deviation (n ≥ 3, unless indicated otherwise)