Table 5.
Physicochemical properties of NC-mRNA nanocarriers, using different types of mRNA, polymers and RNA to polymer ratios
| Code | Type of mRNA | RNA: polymer ratio (w/w) | Size (nm) | PDI | Z-Pot (mV) | EE (%) |
|---|---|---|---|---|---|---|
| NC-3-PR | mGFP | 1:1 | 152 ± 9 | 0.12 ± 0.01 | + 24 ± 1 | 100 |
| NC-4-PR | mGFP | 1:1 | 153 ± 16 | 0.11 ± 0.03 | + 27 ± 2 | 75 |
| NC-3-DX | mGFP | 1:1 | 144 | 0.09 | -26 | 100 |
| NC-4-DX | mLuc | 1:1 | 154 ± 8 | 0.14 ± 0.04 | -14 ± 1 | 100 |
| 1:2 | 126 ± 3 | 0.11 ± 0.02 | -21 ± 2 | 100 | ||
| mOVA | 1:2 | 132 ± 3 | 0.07 ± 0.03 | -16 ± 2 | 100 | |
| NC-5-DXm | mGFP | 1:2 | 93 ± 9 | 0.2 ± 0.01 | -6 ± 7 | 100 |
| NC-5-PPm | mGFP | 1:12 | 70 ± 1 | 0.17 ± 0.01 | + 13 ± 5 | - |
Abbreviations: CS: chitosan. DX: dextran sulphate. EE: encapsulation efficiency. m: base NE-mRNA prepared by microfluidics. mGFP: mRNA encoding for GFP. mLuc: mRNA encoding for luciferase. mOVA: mRNA encoding ovalbumin protein. NC: nanocapsule. PDI: polydispersity index. PP: PEG-PGA or PEG (5 kDa)-b-PGA (10) (Na). PR: protamine sulphate EP. w/w ratio: weight-to-weight ratio between polymer and mRNA coating. Values represent the mean ± standard deviation (n ≥ 3, unless indicated otherwise)