Fig. 6. LION/repRNA gH/gL elicits stronger vaccine-specific CD8⁺ T cell responses than the recombinant gH/gL ectodomain.

Exemplary IFNɣ⁺ CD8⁺ (a) and IFNɣ⁺ CD4⁺ (b) T cell responses in unstimulated or gH/gL-stimulated splenocytes from mice immunized with recombinant gH/gL or LION/repRNA gH/gL using an intercellular staining assay as indicated. See Fig. S7 for gating strategy. Frequency of IFNɣ⁺ CD8⁺ T (c) and CD4⁺ T (d) cells in unstimulated and gH/gL-stimulated splenocytes from mice immunized with recombinant gH/gL or LION/repRNA gH/gL as indicated. Each dot represents an individual mouse (n = 10 per group), The bars represent the mean. Significant differences were determined using a Mann-Whitney test in (c) and (d).