FIG. 3.

HR1 is trimeric in solution. (A) A representative data set from the sedimentation equilibrium analysis of HR1, shown as a plot of A₂₈₀ against radial position (150 mM NaCl, 20 mM NaH₂PO₄, pH 7.4). A total of nine data sets were recorded and fitted globally to a range of models. A fit of the data to a monomer↔trimer model is shown. Residuals from the fit of this data set to single-species (B), monomer↔dimer (C), and monomer↔trimer (D) models are also shown, demonstrating that the monomer↔trimer model fits best to the data.