FIG. 4.

NMR data on the HR1 domain of RSV. (A) ¹⁵N-HSQC spectrum of HR1 (1 mM, pH 5.5). Arbitrary peak numbers are indicated on the spectrum. The side chains of Asn and Gln residues, together with aliased signals from Arg and Lys residues, are boxed. The inset panel shows an expansion of the most overlapped region of the spectrum. (B) Graph of ³J_HNHα against peak number, measured from the HNHA spectrum of HR1. The solid line indicates the limiting value of ³J_HNHα below which a residue is very likely to be in a helical conformation.