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. 2024 Jun 17;28(2):320. doi: 10.3892/etm.2024.12609

Figure 4.

Figure 4

Silencing of p53 attenuates the effects of MIR193BHG on HTR-8/SVneo cells. HTR-8/SVneo cells were divided into three groups (MIR193BHG-OE + p53-siRNA-3, MIR193BHG-OE + NC-siRNA and NC-OE + NC-siRNA), after which rescue experiments were performed. (A) Reverse transcription-quantitative PCR and (B) western blotting were performed to examine mRNA and protein expression levels of p53, respectively, following transfection with p53-siRNA-1, p53-siRNA-2 and p53-siRNA-3. ***P<0.001 vs. NC-siRNA. (C) Cell proliferation was measured using a Cell Counting Kit-8 assay and (D) flow cytometry was used to detect apoptosis. (E) Wound healing assays were used to examine cell migration (magnification, x40). (F) A Transwell invasion assay was used to examine cell invasion (magnification, x100). *P<0.05, **P<0.01 and ***P<0.001 vs. NC-OE + NC-siRNA; #P<0.05 vs. MIR193BHG-OE + NC-siRNA. OE, overexpression; NC, negative control; siRNA, small interfering RNA; OD, optical density; NC-OE, empty vector; NC-siRNA, non-targeting siRNA negative control.