Fig. 3.

In vitro intracellular uptake and cuproptosis induction of the ES-Cu-MOF nano-regulator. (A and B) The percentage and mean fluorescence intensity (MFI) of Ce6-positive cells in MCA205 cells were measured by flow cytometry after treatment at different time points. (C) Immunofluorescence detection of cell internalization treatment with Cu-MOF_Ce6 in MCA205 cells. (D) Cell viability of MCA205 cells treated with the indicated concentrations of elesclomol for 6 h. (E) Cell viability of MCA205 cells in various treatments at different time points. (F) The Western blot analysis of FDX1, DLA,T and HSP70 expression following 6-h pulse treatments of various types. (G) Confocal microscopy analysis in MCA205 cells treated with different formulations, followed by staining with MitoSOX and DAPI. (H) The relative fluorescence intensity of mitoSOX/DAPI in MCA205 cells after different treatments. Data presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.