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Factors that affect the development of cell-based potency assays (CBPAs). The development of CBPAs is influenced by many different factors, some examples of which are listed under each step. Variations in these factors influence assay performance. This graphic depicts the general steps in the Allergan/AbbVie CBPA. Modified from Rupp et al. 2021 [108] and Fernandez Salas et al. 2012 [106].
