Figure 3.

Comparison of virus immunocapture with anti-Env antibodies across different virus model systems. Plate-based virion immunocapture assays were performed with wells coated with the antibodies PGT126, 246-D, PG9, or an isotype control. Captured viruses were lysed and HIV-1 p24 Gag was quantified using p24 AlphaLISA as an indicator of the amount of virus capture. (A) Viruses (IIIB and BaL) produced in T cell lines (PM1, Jurkat, H9) were added to the wells at a normalized concentration of input virus (50 ng/mL of viral p24). (B) The HIV_BaL isolate propagated in two different PBMC donors (BaL 1 and BaL 2), and (C) viruses produced from the transfection of infectious molecular clones (IMC; NL4-3 BaL and NL4-3) in HEK293T cells were tested at their undiluted titers. Results represent the mean ± SD of duplicate wells and are representative of three independent experiments.