Table 1.
Quantitative staining data from an anti-Env antibody panel performed on HIVIIIB produced in H9 CD4+ T cells. Quantitative data represent the PE MESF statistics from a gate encompassing all virus events (i.e., the sum of the upper and lower gates in Figure 1) after subtraction of background fluorescence, as measured by two isotype controls. Stains with high intensity are indicated by a darker shade of red, with low intensity stains in yellow, and absent stains unreported and shaded white. Data represent the mean and standard deviation of the median PE MESF values from two independent experiments. * N6/PGDM1400x10E8 is tri-specific antibody.
| HIV Env Trimer Domain |
Antibody | ARP Catalog # |
Mean (PE MESF) |
SD |
|---|---|---|---|---|
| V3 glycan supersite | 257-D IV | 1510 | 0.08 | 0.15 |
| F425 B4e8 | 7626 | - | - | |
| 39F | 11437 | - | - | |
| PGT126 | 12344 | 11.27 | 0.19 | |
| 10065D | 13426 | - | - | |
| 2557 | 13429 | 0.60 | 0.27 | |
| 2558 | 13432 | 1.49 | 0.28 | |
| 2191 | 11682 | 1.98 | 0.34 | |
| 10074 | 12477 | 1.42 | 0.28 | |
| 447-52D | 4030 | 0.65 | 0.10 | |
| F425 B4a1 | 7625 | 0.01 | 0.06 | |
| 3074 | 12040 | - | - | |
| 2G12 | 1476 | 0.62 | 0.12 | |
| 268-D IV | 1511 | - | - | |
| 3869 | 12039 | - | - | |
| PGT128 | 13352 | 10.92 | 0.64 | |
| 2219 | 11683 | - | - | |
| PGT121 | 12343 | 0.74 | 0.16 | |
| 5F7 | 2533 | 0.57 | 0.20 | |
| gp160 | 902 | 522 | 1.16 | 0.34 |
| Chessie 6 | 810 | 0.20 | 0.08 | |
| ID6 | 2343 | 0.46 | 0.14 | |
| gp120 | IgG YZ23 | 12047 | 0.05 | 0.14 |
| Constant region 1 | A32 | 11438 | - | - |
| 654-30D | 7369 | - | - | |
| CH38 | 12548 | - | - | |
| CH57 | 12549 | - | - | |
| CH90 | 12552 | - | - | |
| A32-AAA | 12558 | 0.02 | 0.09 | |
| 16H3 | 12559 | 0.17 | 0.07 | |
| 3B3 | 12560 | 0.16 | 0.08 | |
| Chessie 13-39.1 | 1209 | 0.09 | 0.06 | |
| Constant region 5 | 670-30D | 7370 | 0.06 | 0.06 |
| CD4bs | VRC01 | 12033 | 0.21 | 0.10 |
| 3BNC117 | 12474 | 0.27 | 0.07 | |
| VRC-CH31 | 12565 | - | - | |
| CH106 | 12566 | 0.14 | 0.08 | |
| F105 | 857 | 0.02 | 0.09 | |
| * N6/PGDM1400x10E8 | 13390 | 2.53 | 0.35 | |
| N6 | 12968 | 0.22 | 0.19 | |
| VRC03 | 12032 | 1.19 | 0.22 | |
| NIH45-46 G54W | 12174 | 1.85 | 0.57 | |
| IgG1 b12 | 2640 | 1.47 | 0.07 | |
| HJ16 | 12138 | 1.52 | 0.09 | |
| CD4i | E51 | 11439 | 2.76 | 0.14 |
| scFv m9 | 11710 | 0.05 | 0.08 | |
| F425 A1g8 | 7624 | 1.07 | 0.37 | |
| 17b | 4091 | 0.81 | 0.14 | |
| 48d | 1756 | 0.95 | 0.23 | |
| gp120 interface | 35022 | 12586 | 0.07 | 0.08 |
| MPER | Z13e1 | 11557 | - | - |
| 10E8 | 12294 | - | - | |
| 7H6 | 12295 | - | - | |
| 10E8v4 | 12865 | - | - | |
| 2F5 | 1475 | - | - | |
| 4E10 | 10091 | - | - | |
| V1V2 | CH58 | 12550 | - | - |
| CH59 | 12551 | - | - | |
| HG107 | 12553 | - | - | |
| HG120 | 12554 | - | - | |
| CH01 | 12561 | - | - | |
| CH01 | 12562 | - | - | |
| CH03 | 12563 | - | - | |
| CH04 | 12564 | - | - | |
| PG9 | 12149 | 7.62 | 0.16 | |
| PG16 | 12150 | 2.94 | 0.24 | |
| 2909 | 12141 | - | - | |
| PGT145 | 12703 | 0.08 | 0.07 | |
| 697-30D | 7371 | - | - | |
| Ibalizumab (PG9-iMab) | 12633 | 0.40 | 0.07 | |
| gp41 | 246-D | 1245 | 1.95 | 0.19 |
| 7B2 | 12556 | 1.02 | 0.32 | |
| 7B2-AAA | 12557 | 1.87 | 0.07 | |
| 240-D | 1242 | 1.74 | 0.31 | |
| T32 | 11391 | 0.47 | 0.12 | |
| NC | 11482 | 0.08 | 0.06 | |
| 5F3 | 6882 | 0.01 | 0.18 | |
| F240 | 7623 | 1.97 | 0.12 | |
| D50 | 11393 | 0.11 | 0.08 | |
| 1577 | 1172 | 0.08 | 0.06 | |
| Chessie 8 | 13049 | 0.08 | 0.09 | |
| 167-D IV | 11681 | - | - | |
| 50-69 | 531 | 1.91 | 0.20 | |
| D5 | 12296 | - | - | |
| 126-7 | 9967 | 0.03 | 0.12 |