TABLE 1.
Primers used in this study
| Experiment | Primer name | Primer sequencea |
|---|---|---|
| SgRNA knockout | SG2A/T | CCCAGGATCCGATTTGTGCTAGTGGTGTTGTCTTCACAGGAATTGCGCCTTGTA |
| SG2G/C | CCCAGGATCCGATTTGTGCTAGTGGTGTTGTCTTCACTGGAATTGCGCCTTGTA | |
| SG3G/C | CCACGACCTGGTACAAGT | |
| SG3G/C2 | GAAGACGTTAAAACTCGACCACCTGGTACAAGTCGT | |
| 3′wt | ATACCCCGGGTTGCCGAA | |
| CB0416 | GGTCTAGATAATACGACTCACTATAGGGTACACAAACAAGCGAAT | |
| SgRNA2 promoter deletion | 4620D | CCTGAAGACGTACCTCCAAT_AAAGAAGAACCCCCA |
| 4686D | TCCACGCTACTCTATGAAAG_CTACTCCCACTGCC | |
| 4706D | GGCAACTTTTTATCCAGACT_GTGTCAACTACTTCAAACAT | |
| 4729D | CCAGACTTGTAGAAGCGA_AAGGGAGCAGCTCCG | |
| 4763D | ACTCCCACTGCCCCATCC_AATTCCAGCGGAATC | |
| 4790D | CAAACATGACAAGGGAGC_GCGTACAAGGCGCAA | |
| 4810D | CCGGGAGTACACTAGGATT_GTGAAGACAACACCAC | |
| 4811D | CCGGGAGTACACTAGGATT_TGAAGACAACACCACTA | |
| P018 | GCCTGTTTCCCAGGATCCTATAGTGAGTCGTATTACA | |
| Promoter duplication | 4620 | ATAGGTACCAAAGAAGAACCCCA |
| 4686 | ATAGGTACCCTACTCCCACTGCC | |
| 4810 | ATAGGTACCGTGAAGACAACACCACTA | |
| 4900 | ATAGGTACCACGGCGGTAGGTTG | |
| 4922 | ATAGGTACCCATCGGCCAAACACAATA | |
| 4952 | ATAGGTACCAATACAAACGGCGA | |
| 5249 | ATAGGTACCAAGGCTATCCCACC | |
| 5293 | ATAGGTACCAGTGGGTGACTTCG | |
| 5319 | ATAGGTACCGATCGTCAGGATTGA | |
| 5330 | ATAGGTACCTTGAAGACGTTAAACTC | |
| 5345 | ATAGGTACCCTCGACGACCTGGTACAA | |
| 5373 | ATAGGTACCAGTTTAACGACTTGTACCA | |
| 5388 | ATAGGTACCGTATCCACCCGAGTC | |
| 5402 | ATAGGTACCGGGCCGGGTGTGGTG | |
| 5432 | ATAGGTACCCGTTTCGTATCGTG |
a
Altered bases are in boldface, deletions are shown as underlined spaces, and KpnI recognition sites are underlined.