Fig. 6.

Wounding of Arabidopsis inflorescence stems does not trigger wax biosynthesis. (A) Expression of genes involved in wax biosynthesis before (0 h) and after wounding (2 and 5 h) in WT and the JA-Ile-deficient mutant dde2-2. The transcript was analyzed by qRT-PCR. Quantification of (B) JA-Ile and( C) ABA in non-wounded (0 h) and wounded (2 and 5 h) inflorescence stems of WT and dde2-2 by LC-MS/MS. (D) Wax load of non-wounded (0 h), wounded and immediately extracted (R) inflorescence stems as well as stems harvested 4, 6 or 8 h post wounding were analyzed by GC-FID. Values represent means (±SD) of analyses from three independent experiments. Letters indicate statistical significance determined by ANOVA and Tukey’s post-hoc test (P < 0.05). Data of this figure are available in Supplementary Table S8.