Figure 1.

tsRNA-1599 is a potential regulator of experimental neovascularization. (A) Volcano plot filtering was conducted to identify differentially expressed tsRNAs in the RPE-choroid-sclera complexes between CNV group and non-CNV group. (B and C) Length distribution of tsRNAs were shown in non-CNV group (B) and CNV group (C). (D and E) Stacked plots displaying the percentage of each tsRNA sub-type sorted by the sites and length expressed in non-CNV group (D) and CNV group (E). (F) Pie charts showing the percentage of different tsRNA types in non-CNV group and CNV group. (G) qRT-PCR assays were conducted to compare the expression of the indicated tsRNAs between CNV group and non-CNV group (n = 4, *P < 0.05 vs. non-CNV, Student t test). (H) HUVECs were treated with CoCl₂ (300 μmol/L) to mimic hypoxic condition or left untreated (Ctrl) for 24 h. qRT-PCR assays were conducted to detect the expression of the indicated tsRNAs in HUVECs (n = 3, *P < 0.05 vs. Ctrl, Student t test).