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. 2024 Jun 1;14(9):3509–3525. doi: 10.7150/thno.96946

Figure 2.

Figure 2

tsRNA-1599 expression is significantly up-regulated in experimental neovascularization in vitro and in vivo. (A) The expression levels of nucleus control transcript (U6), cytoplasm control transcript (β-actin), and tsRNA-1599 were detected by qRT-PCR assays in the nucleus and cytoplasm fraction of HUVECs (n = 3). (B) RNA-FISH assays were conducted to detect the distribution of tsRNA-1599 expression in HUVECs using Cy3-labeled probe (tsRNA-1599). Nucleus control transcript (U6) and cytoplasm control transcript (18S rRNA) was also detected. Nuclei were stained with 4ʹ, 6-diamidino-2-phenylindole (DAPI). Scale bar, 10 μm. (C) qRT-PCRs were conducted to detect the levels of tsRNA-1599 expression in the RPE-choroid-sclera complexes of C57BL/6J mice after 3, 5, 7, 14-day laser photocoagulation (n = 3, *P < 0.05 vs. Ctrl, One-way ANOVA followed by Bonferroni's post hoc test). (D) qRT-PCR assays were conducted to compare the levels of tsRNA-1599 expression in the OIR retinas and normal retinas (n = 3, *P < 0.05 vs. Ctrl, Student t test). (E) qRT-PCR assays were conducted to compare tsRNA-1599 expression in DR retinas and non-DR retinas (n = 3, *P < 0.05 vs. Ctrl, Student t test). (F) qRT-PCR assays were conducted to compare tsRNA-1599 expression in aqueous humor of nAMD patients (n = 8) or DR patients (n = 8) with age related cataract (ARC, n = 7 vs. ARC, *P < 0.05, One-way ANOVA followed by Bonferroni's post hoc test).