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. 2024 Jun 1;14(9):3509–3525. doi: 10.7150/thno.96946

Figure 4.

Figure 4

tsRNA-1599 silencing plays an anti-angiogenic role in experimental angiogenesis. (A) IB4 staining of the whole-mount retinas from OIR mice injected without or with aflibercept (40 mg/mL, 1 μL), scramble (Scr) antagomir (20 μM, 1 μL), or tsRNA-1599 antagomir (20 μM, 1μL) at P17 (n = 5 mice for each group, Scale bar, 500 μm) along with the quantification of avascular areas and neovascular tufts (NVTs). White line denotes retinal margin and white area represents NVT. In the insets, red line: retinal margin; blue area: avascular area; red area: NVTs. (B and C) Quantification analysis of avascular areas and NVTs, respectively. *P < 0.05 vs. OIR group; #P < 0.05 between the marked group; One-way ANOVA followed by Bonferroni's post hoc test. (D and E) Retinal trypsin digestion was conducted to detect the number of acellular capillaries in non-DR mice (Ctrl), DR mice, DR mice-injected Scr antagomir (20 μM, 2 μL), tsRNA-1599 antagomir (20 μM, 2 μL), or aflibercept (40 mg/mL, 2 μL). Yellow arrows indicated acellular capillaries (n = 5 mice for each group, Scale bar, 10 μm). *P < 0.05 vs. DR group; #P < 0.05 between the marked group; One-way ANOVA followed by Bonferroni's post hoc test.