Figure 6.

tsRNA-1599 is an indirect regulator of VEGF signaling in endothelial cells. HUVECs were transfected with negative control (NC) mimic (30 nM), tsRNA-1599 mimic (30 nM), NC inhibitor (30 nM, tsRNA-1599 inhibitor (30 nM), or left untreated (Ctrl) for 24 h, and then stimulated with VEGF (100 ng/mL) for up to 2 h. Western blots were conducted to detect the expression levels of VEGFR2, p-VEGFR2, Akt, p-Akt, p38, p-p38, ERK, and p-ERK (n = 3). β-actin was detected as the internal control. “ns” represents no statistical significance; One-way ANOVA followed by Bonferroni's post hoc test.