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. 2024 Jun 1;14(9):3509–3525. doi: 10.7150/thno.96946

Figure 8.

Figure 8

tsRNA-1599 regulates endothelial angiogenic effects by interacting with YBX1. (A) Silver staining of tsRNA-1599-associated proteins following RNA pull-down assays by tsRNA-1599 mimic, scramble tsRNA mimic (NC), and streptavidin beads. (B) Western blot analysis of YBX1 expression following RNA pull-down assays using tsRNA-1599 mimic or scramble tsRNA mimic (NC) (n = 3, *P < 0.05 between the marked group, One-way ANOVA followed by Bonferroni test). (C) RIP-qPCR analysis of tsRNA-1599 expression following RNA immunoprecipitation using anti-YBX1 or IgG in HUVECs (n = 6, *P < 0.05 between the marked group, Student t test). (D) Western blot analysis of YBX1 expression following RNA pull-down assays using tsRNA-1599 agomir or scramble tsRNA agomir (NC) in RPE-choroid-sclera complex (n = 3, *P < 0.05 between the marked group, One-way ANOVA followed by Bonferroni test). (E) Immunostaining assays and RNA-FISH assays were conducted to detect the expression of tsRNA-1599 (red) and YBX1 (green) in HUVECs. (F and G) HUVECs were transfected with NC mimic, tsRNA-1599 mimic, NC inhibitor, or tsRNA-1599 inhibitor for 24 h. qPCR assays (F, n = 3) and western blots (G, n = 4) were conducted to detect the expression of YBX1. *P < 0.05 between the marked group; “ns” indicates no statistical significance; Student t test.