Figure 9.

tsRNA-1599 regulates HK2 expression via interacting with YBX1. (A-B) HUVECs were transfected with negative control (NC) mimic (30 nM), tsRNA-1599 mimic (30 nM), NC inhibitor (30 nM), or tsRNA-1599 inhibitor (30 nM) for 48 h. qPCR assays (A, n = 3) and western blots were conducted to detect the expression of selected genes (B, n = 4). *P < 0.05 between the marked group; Student t test; “ns” represents no statistical significance. (C) The predicted result of binding sites between YBX1 and HK2 on the JASPAR website. (D) ChIP-qPCR results of the predicted binding sites (n = 4, *P < 0.05 between the marked group; “ns” represents no statistical significance; Student t test). (E - H) HUVECs were transfected with NC siRNA or YBX1 siRNA for 24 h. qPCR assays (E, n = 3, One-way ANOVA followed by Bonferroni's post hoc test, Scale bar, 20 μm) and western blots were conducted to detect the expression of YBX1 or HK2 (F - G, n = 4, Student t test, *P < 0.05 between the marked group). (H) qPCR assays were used to verify the expression of HK2 (n = 3, *P < 0.05 vs. NC group, Student t test). (I - J) HUVECs were transfected with negative control (NC) mimic (30 nM) plus NC siRNA (30 nM), NC mimic (30 nM) plus YBX1 siRNA (30 nM), NC siRNA (30 nM) plus tsRNA-1599 mimic (30 nM), YBX1 siRNA (30 nM) plus tsRNA-1599 mimic (30 nM) for 48 h. qPCR assays (I, n = 3) and western blots (J, n = 4) were conducted to detect the expression of HK2. *P < 0.05 between the marked group, Student t test.