Figure 3.

(A) Flow cytometric analysis of HTP-BM and HTP-BM/CF uptake by 4T1 cells and quantitative fluorescence statistics (B). (C) In vivo fluorescence imaging of HTP-BM-Cy5 and HTP-BM/CF-Cy5 in mice and ex-vivo isolated tumors and major organs 48 h after tail vein injection. Quantitative fluorescence statistics of in vivo imaging in major organs (D) and tumor along the time (E) in mice. (F) Fluorescence images of 4T1 and 3T3 cells co-incubated with PBS, CFN and CFN (10 μL NTR, 2 μL NADH) for 6 h, respectively (scale bar is 100 μm). G) Fluorescence images of 4T1 cells incubated with CFN and HTP-BM/CFN with or without 10 min laser irradiation (660 nm, 1.2 W/cm²), (-) representing without irradiation, (+) representing with irradiation, scale bar: 20 μm. (H) Dissolved oxygen content in PBS and HTP solutions before and after laser irradiation (660 nm, 1.2 W/cm2). (I) Nitroreductase activity in 4T1 cells in the presence and absence of HTP-BM/CF after irradiation treatment with the time. (J) Nitroreductase activity in 4T1 cells and 3T3 cells in the presence and absence of HTP-BM/CF before and after 30 min of irradiation treatment. (* p<0.05, and **** p<0.0001)