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. 2024 Jun 17;14(9):3739–3759. doi: 10.7150/thno.97412

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ROS scavenging analysis of the 2Mg@PEG-PLGA hydrogel. (A) Schematic illustration of ·OH reacting with H₂ generated from the 2Mg@PEG-PLGA hydrogel. (B) The ·OH scavenging effect of 2Mg@PEG-PLGA detected by ESR. (C) Viability of H₂O₂-stimulated MEFs and RAW264.7 cells without or with hydrogel treatment. (D) ROS scavenging in MEFs and RAW264.7 cells after different treatments. (E-F) FCM results showing the intracellular ROS levels in MEFs and RAW264.7 cells after different treatments and (G) the corresponding quantitative analysis. The data are expressed as the mean ± SD (n=3). n.s. indicates no significance. *p < 0.05, **p < 0.01 and ***p < 0.001.