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. 2024 Jun 17;14(9):3739–3759. doi: 10.7150/thno.97412

Figure 5.

Figure 5

In vitro antiosteoclastic and pro-osteoblastic properties of the 2Mg@PEG-PLGA hydrogel. (A) TRAP staining of RAW264.7 cells cultured in OCM supplemented with or without different hydrogels for 7 days and (B) corresponding quantitative analysis of TRAP+ cells per well. (C) ALP staining and (D) ALP activity quantitative analysis of MEFs after culture with OBM with or without supplementation with different hydrogels for 7 days. (E-F) ARS staining and corresponding quantitative analysis of MEFs after culture with OBM supplemented with or without different hydrogels for 14 days. The data are expressed as the mean ± SD. **p < 0.01 and ***p < 0.001, compared with the control group; ###p<0.001, compared with the OCM (Figure 5B) or OBM (Figure 5D and Figure 5F) group.