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WI-38 and primary lung fibroblasts from idiopathic pulmonary fibrosis patients (LF-IPF) display similar phenotypical and gene expression response to TGF-β1 and Metformin treatment. (A) Experimental approach. (B) details on the 4 different LF-IPF used for the study. (C) IF for LipidTox™. (D) IF for ACTA2. (E) IF for MYH11. (F) qPCR for Metformin vs. vehicle WI-38 and LF-IPF samples. (G) qPCR for TGF-β1 vs. vehicle WI-38 and LF-IPF samples. Scale bar C-E: 50 μm.
