Figure 4.

Bulk RNA-Seq analysis of W-I38 and LF-IPF treated with Metformin or TGF-β1 indicate similar transcriptomic changes. (A) Experimental approach. (B) PCA analysis FIB n = 2, LIF n = 3, MYF n = 2. (C) Comparison of Metformin vs Vehicle treatment for WI-38 and LF-IPF using historical data ³¹. n = 3. (D) Comparison of TGF-β1 vs Vehicle treatment for WI-38 and LF-IPF using historical data ³². n = 3. (E) Top 35 genes simultaneously upregulated by TGF-β1 and downregulated by Metformin (F) Top 35 genes simultaneously downregulated by TGF-β1 and upregulated by Metformin.