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. 2024 Jun 11;14(9):3603–3622. doi: 10.7150/thno.93519

Table 1.

Primer sequences used for qPCR analysis.

Gene Forward primer sequence Reverse primer sequence
ACTA2 5' -CTG TTG CAG CCA TCC TTC AT- 3' 5' -TCA TGA TGC TGT TGT AGG TGG- 3'
ADRP 5' -TCA GCT CCA TTC TAC TGT TCA CC- 3' 5' -CCT GAA TTT TCT GAT TGG CAC- 3'
COL1A1 5' -ATG TTC AGC TTT GTGGAC CTC- 3' 5' -CTG TAC GCA GGT GAT TGG TG- 3'
PPARγ 5' -TTG CTG TCA TTA TTC TCA GTG GA- 3' 5' -GAG GAC TCA GGG TGG TTC AG- 3'
MYH11 5' -GCA GCT ACA GGC TGA AAG GA- 3' 5' -CCT CCA GCT GTT CTT CAA GG- 3'
GAPDH 5' -GAA AGC CTG CCG GTG ACT AA- 3' 5' -GCC CAA TAC GAC CAA ATC AGA G- 3'

Table 1: List of the forward and reverse primers used in the qPCR analysis: ACTA2, COL1A, and MYH11 as MYF markers; ADPR, and PPARγ as LIF markers. GAPDH serves as a housekeeping gene.