Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CCT1 | Santa Cruz Biotechnology | Cat # sc-374088 RRID:AB_10918262 |
| CCT1 | Abcam | Cat# ab240903 RRID:AB_2920800 |
| CCT2 | Abcam | Cat # ab92746 RRID:AB_10565196 |
| CCT2 (rabbit) | 81 | N/A |
| CCT3 | Abcam | Cat # ab106932 RRID:AB_10865346 |
| CCT3 | Santa Cruz Biotechnology | Cat # sc-271336 RRID:AB_10610107 |
| CCT4 | Santa Cruz Biotechnology | Cat # sc-137092 RRID:AB_2073768 |
| CCT5 | Abcam | Cat # ab129016 RRID:AB_11154964 |
| CCT5 (rabbit) | 81 | N/A |
| CCT6 | Santa Cruz Biotechnology | Cat # sc-514466 RRID:AB_11154964 |
| CCT7 | Santa Cruz Biotechnology | Cat # sc-271951 RRID:AB_10709576 |
| CCT8 | Santa Cruz Biotechnology | Cat # sc-377261 RRID:AB_2917991 |
| IRDye 680RD Donkey anti-rabbit IgG | LI-COR Biosciences | Cat# 926-68073 RRID:AB_10954442 |
| IRDye 800CW Donkey anti-mouse IgG | LI-COR Biosciences | Cat# 926-32212 RRID:AB_621847 |
| IRDye 800CW Donkey anti-Goat IgG (H + L) | LI-COR Biosciences | Cat# 926-32214 RRID:AB_621846 |
| IRDye 800CW Goat anti-Rat IgG (H + L) | LI-COR Biosciences | Cat# 926-32219 RRID:AB_1850025 |
| Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson Immuno Research | Cat# 711-035-152 RRID:AB_10015282 |
| Peroxidase AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson Immuno Research | Cat# 715-035-151 RRID:AB_2340771 |
| Peroxidase AffiniPure Donkey Anti-Goat IgG (H+L) | Jackson Immuno Research | Cat# 705-035-003 RRID:AB_2340390 |
| Anti-GFP-MBP nanobody | Modified by MPB addition, this study | N/A |
| Bacterial and virus strains | ||
| E. coli strain DH10Bac | Thermo Fisher | Cat# 10361012 |
| Chemicals, peptides, and recombinant proteins | ||
| HCCCT578631 | This study | N/A |
| HCCCT1368 | This study | N/A |
| HCCCT4257 | This study | N/A |
| HCCCT57 | This study | N/A |
| TRIC | Collier et al., 2021 | N/A |
| DMEM supplemented with glucose | Thermo Fisher Scientific | Cat# 10566024 |
| Fetal Bovine Serum | Thermo Fisher Scientific | Cat# 26400044 |
| DharmaFECT #4 transfection reagent | DharmaFECT | Cat# T-2004-03 |
| DharmaFECT Cell Culture Reagent (DCCR) | Thermo Fisher Scientific | Cat# B-004500-100 |
| Complete, EDTA-free Protease Inhibitor Cocktail | Roche | Cat# 4693132001 |
| Lipofectamine™ 2000 | Thermo Fisher Scientific | Cat# 11668019 |
| Z-Leu-Leu-Leu-al | Sigma-Aldrich | Cat# C2211-5MG |
| Ammonium Chloride | Sigma-Aldrich | Cat# A9434 |
| Benzonase® Nuclease | Sigma-Aldrich | Cat# E1014-5KU |
| Ni Sepharose® High Performance | Sigma-Aldrich | Cat# GE17-5268-02 |
| Calmodulin Sepharose™ 4B | VWR | Cat# 95016-874 |
| Amylose Resin | New England Biolabs | Cat# E8021S |
| DSS-H12/D12 | Creatie Molecules Inc. | Cat# 001S |
| Adenosine 5’-triphosphate disodium salt hydrate | Sigma-Adlrich | Cat# A7699 |
| DNaseI | Worthington Biochemical | Cat# LS006333 |
| DNaseI | Sigma-Aldrich | Cat# D5025 |
| Critical commercial assays | ||
| Quick-RNA miniprep Kit | Zymo Research | Cat# R1054 |
| iScript cDNA Synthesis Kit | Bio-Rad | Cat# 1708891 |
| Itaq™ Universal SYBR assay | Bio-Rad | Cat# 1708891 |
| [35S] EasyTag™ express protein labeling mix | Perkin Elmer | Cat# NEG772002MC |
| Deposited data | ||
| Unprocessed western blot deposited in Mendeley Data | This paper | DOI: 10.17632/ymrbcv2wrt.1 |
| Unprocessed images of negative stain | This paper | DOI: 10.17632/ymrbcv2wrt.1 |
| XL-MS raw data in Mendeley Data | This paper | DOI: 10.17632/ymrbcv2wrt.1 |
| Map of HCCCT578631 | This paper | EMD-32393 |
| Coordinates of the TRiC in open conformation | Gestaut et al. 2022 | PDB:7WU7 |
| Experimental models: Cell lines | ||
| Hela cells (CCL-2) | ATCC | CCL-2 |
| SF9 cells | Thermo Fisher Scientific | 12659-017 |
| High Five cells (Hi5) | Invitrogen | B85502 |
| Oligonucleotides | ||
| Primers used in this study are listed in Table S1 | This paper | N/A |
| Recombinant DNA | ||
| pFB-Duet CCT7His, CCT5 | Gestaut et al., 2019 | pDG463 |
| pFB-Duet CCT2, CCT4, | Gestaut et al., 2019 | pDG443 |
| pFB-Duet CCT6, CCT3 | Gestaut et al., 2019 | pDG445 |
| pFB-Duet CCT8, CCT1 | Gestaut et al., 2019 | pDG446 |
| pFB-Duet CCT1CBP, CCT8 | Gestaut et al., 2019 | pDG518 |
| pFB-Duet CCT1GFP, CCT8 | Gestaut et al., 2019 | pDG517 |
| pFB-Duet HIS-ACTB | This study | pYC26 |
| pcDNA3.1 CCT2-Ch C-terminal mCherry | (Zhao et al., 2016) | pJF1919 |
| pcDNA3.1 CCT4-Ch C-terminal mCherry | (Zhao et al., 2016) | pJF1921 |
| pcDNA3.1 CCT5-Ch C-terminal mCherry | (Zhao et al., 2016) | pJF1922 |
| pCMV6 CCT1 | Eric Jonasch | N/A |
| pCMV6 CCT2 | Eric Jonasch | N/A |
| pCMV6 CCT3 | Eric Jonasch | N/A |
| pCMV6 CCT4 | Eric Jonasch | N/A |
| pCMV6 CCT5 | Eric Jonasch | N/A |
| pCMV6 CCT6 | Eric Jonasch | N/A |
| pCMV6 CCT7 | Eric Jonasch | N/A |
| pcDNA YFP | Addgene | #13033 |
| siGENOME Human TCP1 (6950) siRNA - SMARTpool | Thermo Scientific | M-012749-01-0005 |
| siGENOME Human CCT2 (10576) siRNA - SMARTpool | Thermo Scientific | M-020107-02-0005 |
| siGENOME Human CCT3 (7203) siRNA - SMARTpool | Thermo Scientific | M-018339-01-0005 |
| siGENOME Human CCT4 (10575) siRNA - SMARTpool | Thermo Scientific | M-020147-00-0005 |
| siGENOME Human CCT5 (22948) siRNA - SMARTpool | Thermo Scientific | M-012797-02-0005 |
| siGENOME Human CCT6A (908) siRNA - SMARTpool | Thermo Scientific | M-016559-01-0005 |
| siGENOME Human CCT7 (10574) siRNA - SMARTpool | Thermo Scientific | M-020115-01-0005 |
| siGENOME Human CCT8 (10694) siRNA - SMARTpool | Thermo Scientific | M-008566-02-0005 |
| siGENOME Non-Targeting siRNA Pool #2 |
Thermo Scientific | D-001206-14-05 |
| Software and algorithms | ||
| ImageJ | Schneider et al. 2021 | https://imagej.nih.gov/ij/index.html |
| GraphPad Prism 7.04 & 9.1.2 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| CFX Maestro Manager | BioRad | https://www.bio-rad.com/en-us/product/cfx-maestro-software-for-cfx-real-time-pcr-instruments?ID=OKZP7E15 |
| Image Studio Lite | Li-COR Biosciences | https://www.licor.com/bio/image-studio-lite/ |
| Pymol 1.8.6.2 | Schrӧdinger | https://pymol.org/2/ |
| UniDec v3.1.0 | Marty et al., 2015 | https://github.com/michaelmarty/UniDec |
| MaCSED v0.3 | Justin Benesch | benesch.chem.ox.ac.uk/resources.html |
| MaxQuant v1.6.14 | Tyanova et al., 2016a | https://maxquant.net/download_asset/perseus/latest |
| Perseus v1.6.5 | Tyanova et al., 2016b | https://maxquant.net/download_asset/perseus/latest |
| cryosparc2 | Punjani et al., 2017 | https://cryosparc.com/ |
| RStudio Versi on 1.2.1335 | The R foundation | https://www.r-project.org/ |
| xQuest | Walzthoeni et al., 2012 | https://bioinformaticshome.com/tools/proteomics/descriptions/xQuest.html |
| UCSF ChimeraX | Pettersen et al., 2021 | https://www.cgl.ucsf.edu/chimerax/ |