FIG. 2.

Effects of HTLV-1-infected lymphocytes on BBB permeability. (A) FITC-dextran (70 kDa) clearance values through GPNT and primary HUVEC monolayers. The clearance (slope of line relating cleared volume to time) was measured for filters without cells (open squares), GPNT cells (solid circles), and primary HUVEC (open circles). Results are for triplicate filters from one representative experiment. (B) Paracellular permeability of GPNT cells exposed to HTLV-1-infected lymphocytes. Clearance values for FITC-dextran (70 kDa) through endothelial monolayers were estimated for GPNT cells alone (solid squares) or GPNT cells following coculture with CEM (open circles) or MT2 (solid circles) cells for 18 h at 37°C. Results are for triplicate filters from one experiment representative of two.