FIG. 5.

Infection of GPNT cells by HTLV-1. (A) PCR and RT-PCR amplifications. tax proviral sequences were detected in endothelial cells cocultivated with irradiated MT2 (lanes 1, 4, and 7) and CH patient lymphocytes (lanes 2, 5, and 8) 30 and 60 days postinfection (lanes 1 and 2 and lanes 4 and 5 respectively) but not at 90 days (lanes 7 and 8). Spliced tax mRNA was detected in GPNT cells cocultivated with irradiated MT2 (lanes 10, 13, and 16) or CH lymphocytes (lanes 11, 14, and 17), at 30 and 60 days postinfection (lanes 10 and 11 and lanes 13 and 14) but not at 90 days (lanes 16 and 17). No PCR (lanes 3, 6, and 9) or RT-PCR (lanes 12, 15, and 18) amplification was detected in coculture with irradiated CEM cells. (B) Release of the viral protein p19 into the culture medium following cocultivation with irradiated MT2 (open squares) or CH (solid circles) lymphoid cells. Results are for duplicate wells of one experiment representative of two.