Figure 2.

A1) 3D Reconstruction of the tumor core showing GFP⁺ MDA-MB-231 cells and auto-fluorescent doxorubicin co-localized in cell nuclei. Doxorubicin concentration was varied from 100 to 0.0001 μm. A2) Graphical representation of normalized Caspase 3/7 activity after 24 h of the doxorubicin treatment. B1) 3D Reconstruction of the entire tumor remaining after 72 h of doxorubicin treatment. The tumors were depth coded to represent the entire tumor volume. B2) Graphical representation of tumor volumes after 72 h of doxorubicin perfusion. C1) Heatmap of selected proteins from the human oncology array performed on perfusates collected from devices treated with 1, 0.1, and 0 μm (non-treated) doxorubicin. C2) Graphical representation of fold-change in protein expression for treated devices as compared to non-treated devices. The dotted line at 1 through the bar graph helps identify protein expressions, which had values close or equal to the non-treated group. Thus, values higher than 1 represented an increase in the protein expression and values lower than 1 depicted decrease in expression as compared to the non-treated group (n = 3 for all, p*** < 0.001, p** < 0.01, p* < 0.05).